2. Recombinant Proteins
  3. Enzymes & Regulators
  4. DFF45/ICAD Protein, Human (His)

DFF45/ICAD Protein, Human (His) is the recombinant human-derived DFF45/ICAD protein, expressed by E. coli, with N-6*His labeled tag.

For research use only. We do not sell to patients.

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Description

DFF45/ICAD Protein, Human (His) is the recombinant human-derived DFF45/ICAD protein, expressed by E. coli, with N-6*His labeled tag.
Background

DFF45/ICAD is an inhibitor of the caspase-activated DNase (DFF40), which regulates DNA fragmentation during apoptosis by binding and inhibiting DFF40 activity.
Species

Human
Source

E. coli
Tag

N-6*His
Accession

AAH07721.1 (M1-T331)
Gene ID
Molecular Construction
N-term
6*His
DFF45 (M1-T331)
Accession # AAH07721.1
C-term
Protein Length

Full Length
Synonyms
rHuDNA fragmentation factor subunit alpha/ DFF45, His; DNA Fragmentation Factor Subunit Alpha; DNA Fragmentation Factor 45 kDa Subunit; DFF-45; Inhibitor of CAD; ICAD; DFFA; DFF1; DFF45; H13
AA Sequence

MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT

Molecular Weight

40-50 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Appearance

Solution.
Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH7.4.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation
SDS (251 KB)

COA (219 KB)

Handling Instructions (2659 KB)

DFF45/ICAD Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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DFF45/ICAD Protein, Human (His)
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