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  3. DCAF16-DDB1 Protein, Human (Active, sf9, Biotinylated)

DCAF16-DDB1 Protein, Human (Active, sf9, Biotinylated)

Cat. No.: HY-P703472
Handling Instructions Technical Support

DCAF16 Protein, a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serves as a crucial substrate recognition element. It plays a central role in ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins, emphasizing its key involvement in protein modification processes, particularly in the regulation of protein ubiquitination. DCAF16/DDB1 Protein, Human (Active, Sf9, Biotinylated) is the recombinant human-derived DCAF16/DDB1, expressed by Sf9 insect cells, with tag Free labeled tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
10 μg In-stock
50 μg In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

DCAF16 Protein, a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serves as a crucial substrate recognition element. It plays a central role in ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins, emphasizing its key involvement in protein modification processes, particularly in the regulation of protein ubiquitination. DCAF16/DDB1 Protein, Human (Active, Sf9, Biotinylated) is the recombinant human-derived DCAF16/DDB1, expressed by Sf9 insect cells, with tag Free labeled tag.

Biological Activity

Active

Species

Human

Source

Sf9 insect cells

Tag

Tag Free

Accession

Q9NXF7 (G2-L216)&Q16531-1 (M1-H1140)

Gene ID

54876&1642

Synonyms
C4orf30
Predicted Molecular Mass
24.8 kDa & 126.9 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

DCAF16-DDB1 Protein, Human (Active, sf9, Biotinylated) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
DCAF16-DDB1 Protein, Human (Active, sf9, Biotinylated)
Cat. No.:
HY-P703472
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