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  4. DCAF16-DDB1-CUL4A-RBX1 Protein, Human (sf9)

DCAF16-DDB1-CUL4A-RBX1 Protein, Human (sf9)

Cat. No.: HY-P703559
Handling Instructions Technical Support

DCAF16 Protein, a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serves as a crucial substrate recognition element. It plays a central role in ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins, emphasizing its key involvement in protein modification processes, particularly in the regulation of protein ubiquitination. DCAF16/DDB1/CUL4A/RBX1 Protein, Human (Sf9) is the recombinant human-derived DCAF16/DDB1/CUL4A/RBX1, expressed by Sf9 insect cells, with tag Free labeled tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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Description

DCAF16 Protein, a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serves as a crucial substrate recognition element. It plays a central role in ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins, emphasizing its key involvement in protein modification processes, particularly in the regulation of protein ubiquitination. DCAF16/DDB1/CUL4A/RBX1 Protein, Human (Sf9) is the recombinant human-derived DCAF16/DDB1/CUL4A/RBX1, expressed by Sf9 insect cells, with tag Free labeled tag.
Species

Human
Source

Sf9 insect cells
Tag

Tag Free
Accession

Q9NXF7 (G2-L216 )&Q16531-1 (M1-H1140)&Q13619-1 (A2-A759)&P62877 (M1-H108)
Gene ID

1642
Synonyms
C4orf30
Predicted Molecular Mass
24.1 kDa & 126.9 kDa & 12.2 kDa & 87.6 kDa
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

DCAF16-DDB1-CUL4A-RBX1 Protein, Human (sf9) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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