DCAF15-DDB1 Protein, Human (sf9, His, StrepⅡ)

DCAF15 serves as a substrate recognition component in the DCX (DCAF15) complex, coordinating the ubiquitination and degradation of target proteins and may affect NK cell function and APC-NK cell interactions. Notably, arylsulfonamide anticancer drugs alter the substrate specificity of the complex, promoting ubiquitination and degradation of RBM39, RBM23, and PRPF39. DCAF15-DDB1 Protein, Human (sf9, His, Strep) is the recombinant human-derived DCAF15-DDB1 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.

DCAF15 functions as the substrate-recognition component within the DCX(DCAF15) complex, a cullin-4-RING E3 ubiquitin-protein ligase complex responsible for ubiquitination and subsequent degradation of target proteins. This complex acts as a regulator of natural killer (NK) cell effector functions, potentially by targeting cohesin subunits SMC1A and SMC3 for ubiquitination and degradation. Additionally, DCAF15 may play a role in the activation of antigen-presenting cells (APC) and their interaction with NK cells. Notably, the substrate specificity of the DCX(DCAF15) complex is altered in the presence of aryl sulfonamide anticancer drugs, such as indisulam or E7820. This alteration promotes the ubiquitination and degradation of splicing factor RBM39, leading to splicing defects and cell death in cancer cell lines. These drugs act as molecular glues, facilitating the binding between DCAF15 and RBM39, and also induce ubiquitination and degradation of RBM23 and PRPF39. The unique properties of aryl sulfonamide anticancer drugs highlight DCAF15's pivotal role in modulating substrate specificity and cellular responses within the DCX(DCAF15) complex.

Incubation of DCAF15/DDB1 for 60 minutes at 37˚C in the presence of Bodipy-ubiquitin, UBE1, Ubch5b and ATP was compared alongside two control reactions with either DCAF15/DDB1 or DCAF15/DDB1&ATP excluded from the reaction. Ubiquitin conjugates were identified by the migration of the Bodipy-ubiquitin band and these were observed only in the presence of ATP and DCAF15/DDB1.

Human

Sf9 insect cells

N-StrepⅡ;N-8*His

Q66K64 (A2-L600)&Q16531-1 (M1-H1140)

90379&1642

Approximately 70 KDa/120 KDa

Greater than 85% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.