2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. D15 Protein, Escherichia phage T5

D15 Protein, Escherichia phage T5 is the recombinant D15, expressed by E. coli , with tag Free labeled tag.

For research use only. We do not sell to patients.

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Description

D15 Protein, Escherichia phage T5 is the recombinant D15, expressed by E. coli , with tag Free labeled tag.
Background

D15 is an enzyme with both 5'-exonucleolytic and structure-specific endonucleolytic activities, catalyzing the hydrolysis of DNA branched nucleic acid molecules and likely playing a role in viral genome replication. It is active on flap structures (branched duplex DNA containing a free single-stranded 5'-end), 5' overhangs, and pseudo-Y structures. The substrates require a free single-stranded 5' end, with endonucleolytic hydrolysis occurring at the junction of double- and single-stranded DNA. This function may be used, for example, to trim such branched molecules generated by Okazaki fragment synthesis during replication. by similar: The enzyme's function resembles that of other nucleases with similar activities.
Species

Others
Source

E. coli
Tag

Tag Free
Accession

P06229 (M1-Q291)
Gene ID

2777611
Protein Length

Full Length
Synonyms
exo5
Predicted Molecular Mass
33.5 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

D15 Protein, Escherichia phage T5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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D15 Protein, Escherichia phage T5
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