CXCR5/CD185 Protein-VLP, Human (HEK293)

CXCR5/CD185 Protein-VLP, Human (HEK293) is the recombinant human-derived CXCR5/CD185 protein, expressed by HEK293, with C-His tag.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433. Tags can only be detected under denaturing conditions.

CXCR5 is a cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). It is involved in B-cell migration into B-cell follicles of the spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. It may have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.

Human

HEK293

C-His;Tag Free

P32302-1 (N2-F372)

643

Full Length of Isoform-1

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.