CREG1/CREG Protein, Human (HEK293, His)

CREG1/CREG proteins may contribute to the transcriptional control of cell growth and differentiation and play a key role in counteracting cellular transformation induced by the adenovirus E1A protein. Its activity depends on interaction with IGF2R, functioning as a glycosylation-dependent homodimer. CREG1/CREG Protein, Human (HEK293, His) is the recombinant human-derived CREG1/CREG protein, expressed by HEK293 , with C-6*His labeled tag.

The CREG1/CREG protein emerges as a potential contributor to the transcriptional control of cell growth and differentiation. It plays a pivotal role in antagonizing transcriptional activation and countering cellular transformation induced by the adenovirus E1A protein. Moreover, the transcriptional control activity related to cell growth is contingent on the interaction between CREG1/CREG and IGF2R. Operating as a homodimer, CREG1/CREG also exhibits an interaction with IGF2R that is glycosylation-dependent. These findings suggest a multifaceted role for CREG1/CREG in regulating cellular processes, shedding light on its involvement in transcriptional control mechanisms and its interactions with key cellular components like IGF2R. A more in-depth exploration of these interactions may provide valuable insights into the nuanced molecular mechanisms governing cell growth and differentiation.

Measured by its binding ability in a functional ELISA. Immobilized IGF2R at 2 μg/mL (100μL/well) can bind Biotinylated Human CREG Protein. The ED₅₀ for this effect is 9.563 ng/mL.

Human

HEK293

C-6*His

O75629 (R32-Q220)

8804  [NCBI]

Full Length of Mature Protein

RGGRDHGDWDEASRLPPLPPREDAARVARFVTHVSDWGALATISTLEAVRGRPFADVLSLSDGPPGAGSGVPYFYLSPLQLSVSNLQENPYATLTMTLAQTNFCKKHGFDPQSPLCVHIMLSGTVTKVNETEMDIAKHSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPEEYYNVTVQ

Approximately 25-38 kDa,based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.