CRBN Protein, Human (His)

CRBN Protein, Human (His) is the recombinant human-derived CRBN, expressed by E. coli , with C-6*His labeled tag.

CRBN serves as the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins like MEIS2, ILF2, or GLUL. Its proper degradation of key regulatory proteins is essential for normal limb outgrowth and fibroblast growth factor FGF8 expression. CRBN maintains presynaptic glutamate release and cognitive functions (e.g., memory and learning) by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. It likely functions by modulating BK channel assembly and neuronal surface expression via interaction with KCNT1. By similarity, CRBN may also regulate anxiety-like behaviors through a BK channel-independent mechanism. In TLR4 signaling, CRBN acts as a negative regulator by binding TRAF6 and ECSIT, thereby inhibiting ECSIT ubiquitination—a critical step in the pathway.

Human

E. coli

C-6*His

Q96SW2-1 (M1-L442)

51185

Full Length of Isoform-1

Approximately 56-65 kDa

Greater than 90 % as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.