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  4. CRBN-DDB1-CUL4A-RBX1 Complex Protein, Human (sf9, His, Avi)

CRBN-DDB1-CUL4A-RBX1 Complex Protein, Human (sf9, His, Avi)

Cat. No.: HY-P702826
Handling Instructions Technical Support

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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50 μg In-stock
100 μg   Get quote  
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  • Technical Parameters

  • Properties

  • Documentation

Species

Human
Source

Sf9 insect cells
Tag

N-His;N-Avi
Accession

Q96SW2-1 (M1-L442)&Q16531-1 (M1-H1140)&Q13619-1 (A2-A759)&P62877 (M1-H108)
Gene ID

51185&1642&8451&9978
Synonyms
Cereblon-DDB1-Cul4A-Rbx1 Complex
Molecular Weight

55.3 kDa (CRBN)&126.9 kDa (DDB1)&87.6 kDa (Cul4A)&12.2 kDa (Rbx1)
Purity

Greater than 90 % as determined by reducing SDS-PAGE.
Appearance

Solution.
Formulation

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Endotoxin Level

/
Reconstitution

Please use rapid thawing with running water to thaw the protein.
Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation
Handling Instructions (2659 KB)

CRBN-DDB1-CUL4A-RBX1 Complex Protein, Human (sf9, His, Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CRBN-DDB1-CUL4A-RBX1 Complex Protein, Human (sf9, His, Avi)
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