Copine-1/CPNE1 Protein, Human (His)

Copine-1/CPNE1 protein is a calcium-dependent phospholipid-binding protein that participates in the TNF-α receptor signaling pathway in a calcium-dependent manner. CPNE1 induces AKT-dependent neurite outgrowth and is also involved in TNF-α-induced repression of NF-κ-B transcription. Copine-1/CPNE1 Protein, Human (His) is the recombinant human-derived Copine-1/CPNE1 protein, expressed by E. coli , with N-6*His, C-6*His labeled tag.

Copine-1, also known as CPNE1, is a calcium-dependent phospholipid-binding protein that actively participates in calcium-mediated intracellular processes. Involved in the TNF-alpha receptor signaling pathway, Copine-1 exhibits calcium-dependent phospholipid binding properties. Additionally, it plays a pivotal role in neuronal progenitor cell differentiation by inducing neurite outgrowth through an AKT-dependent signaling cascade, operating in a calcium-independent manner. Copine-1 may also contribute to the recruitment of target proteins to the cell membrane in a calcium-dependent fashion and is implicated in membrane trafficking. Furthermore, it is involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of various NF-kappa-B subunits, including p65/RELA, p50/NFKB1, p52/NFKB2, RELB, and REL. The multifaceted functions of Copine-1 highlight its versatile role in cellular processes, ranging from neuronal development to membrane dynamics and signal transduction pathways.

Human

E. coli

N-6*His;C-6*His

Q99829 (M1-A537)

8904  [NCBI]

Full Length

MAHCVTLVQLSISCDHLIDKDIGSKSDPLCVLLQDVGGGSWAELGRTERVRNCSSPEFSKTLQLEYRFETVQKLRFGIYDIDNKTPELRDDDFLGGAECSLGQIVSSQVLTLPLMLKPGKPAGRGTITVSAQELKDNRVVTMEVEARNLDKKDFLGKSDPFLEFFRQGDGKWHLVYRSEVIKNNLNPTWKRFSVPVQHFCGGNPSTPIQVQCSDYDSDGSHDLIGTFHTSLAQLQAVPAEFECIHPEKQQKKKSYKNSGTIRVKICRVETEYSFLDYVMGGCQINFTVGVDFTGSNGDPSSPDSLHYLSPTGVNEYLMALWSVGSVVQDYDSDKLFPAFGFGAQVPPDWQVSHEFALNFNPSNPYCAGIQGIVDAYRQALPQVRLYGPTNFAPIINHVARFAAQAAHQGTASQYFMLLLLTDGAVTDVEATREAVVRASNLPMSVIIVGVGGADFEAMEQLDADGGPLHTRSGQAAARDIVQFVPYRRFQNAPREALAQTVLAEVPTQLVSYFRAQGWAPLKPLPPSAKDPAQAPQA

58-66 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB，150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.