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  4. Cope1 Protein, Mouse (sf9, His, Strep)

Cope1 Protein, Mouse (sf9, His, Strep) is the recombinant mouse-derived Cope1 protein, expressed by Sf9 insect cells, with Strep & His tag.

For research use only. We do not sell to patients.

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Description

Cope1 Protein, Mouse (sf9, His, Strep) is the recombinant mouse-derived Cope1 protein, expressed by Sf9 insect cells, with Strep & His tag.
Background

Cope1 is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It mediates biosynthetic protein transport from the ER, via the Golgi apparatus, up to the trans Golgi network. The complex is required for budding from Golgi membranes and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, Cope1 can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins. The complex also influences Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).
Species

Mouse
Source

Sf9 insect cells
Tag

Strep;His
Accession

O89079 (M1-A308)
Gene ID

59042
Protein Length

Full Length
Synonyms
Cope; Coatomer subunit epsilon
Predicted Molecular Mass
37.8 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Cope1 Protein, Mouse (sf9, His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Cope1 Protein, Mouse (sf9, His, Strep)
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