Complement factor I Protein, Human (HEK293, His)

Complement factor I Protein, Human (HEK293, His) is the recombinant human-derived Complement factor I protein, expressed by HEK293, with C-His tag.

Complement factor I is a trypsin-like serine protease that plays a crucial role in regulating the immune response by controlling all complement pathways. It inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b, thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase, as well as membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows CFI to degrade deposited C3b, preventing undesired complement activation, while their absence in apoptotic cells or microbes leads to C3b-mediated complement activation and subsequent opsonization.

Human

HEK293

C-8*His

P05156 (K19-V583)

3426

Full Length of Mature Protein

Approximately 75-95 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 90% as determined by Bis-Tris PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.