Complement Factor D/CFP Protein, Human (HEK293, His)

Properdin is a key regulator in the alternative pathway of complement activation, enhancing stability by binding to the C3 and C5 convertase complexes. It prevents CFI-CFH-mediated degradation of C3b to form plasma dimers, trimers, or tetramers. Complement Factor D/CFP Protein, Human (HEK293, His) is the recombinant human-derived Complement Factor D/CFP protein, expressed by HEK293, with C-His labeled tag.

Properdin is a crucial positive regulator within the alternate pathway (AP) of complement activation, playing a pivotal role in enhancing the stability of the C3- and C5-convertase enzyme complexes. By binding to these complexes, Properdin inhibits the CFI-CFH mediated degradation of Complement C3 beta chain (C3b). In its plasma form, Properdin forms dimers, trimers, or tetramers in specific proportions. Notably, Properdin interacts with the pro-C3-convertase enzyme complex (C3b-Bb), consisting of Complement C3 beta chain (C3b) and the Complement factor B Bb fragment (Bb), through its TSP type-1 5 domain. This interaction is pivotal for stabilizing the complex, transforming it into the active C3-convertase enzyme complex (C3b-Bb-FP). Furthermore, Properdin interacts directly with C3b and Complement factor B (CFB), highlighting its multifaceted role in complement activation and regulation.

Human

HEK293

C-His

P27918-1 (D28-L469)

5199

Full Length of Isoform-1 Mature Protein

Approximately 55 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.