Complement Factor B/CFB Protein, Human (HEK293, His)

Complement Factor B/CFB Protein, Human (HEK293, His) is the recombinant human-derived Complement Factor B/CFB protein, expressed by HEK293, with C-6*His labeled tag.

Complement factor B (CFB) is part of the replacement pathway of the complement system and can be cut by factor D into two fragments: Ba and Bb. Bb is a serine protease, which then binds to complement factor 3b to produce C3 or C5 invertase, and is also associated with the proliferation and differentiation of pre-activated b lymphocytes, rapid diffusion of peripheral mononuclear cells, stimulation of lymphoblastogenesis, and lysis of red blood cells. Ba inhibits the proliferation of preactivated b lymphocytes. Activation of the complement replacement pathway (AP) is thought to play an important role in immunoglobulin A nephropathy (IgAN). CFB can inhibit the activity of AP. The expression of CFB was positively correlated with T cell CD8, macrophage M1, plasma cells, T cell CD4 memory activation, T cell follicular assist, and T cell regulation (Tregs). It was negatively correlated with eosinophils, macrophages M0, macrophages M2, mast cell rest and T cell CD4 memory rest in TME. CFB regulates cellular senescence and is associated with poor prognosis in pancreatic cancer^{[1][2][3][4][5][6]}.

Human

HEK293

C-6*His

AAH04143.1 (T26-L764)

629  [NCBI]

Partial

TPWSLARPQGSCSLEGVEIKGGSFRLLQEGQALEYVCPSGFYPYPVQTRTCRSTGSWSTLKTQDQKTVRKAECRAIHCPRPHDFENGEYWPRSPYYNVSDEISFHCYDGYTLRGSANRTCQVNGRWSGQTAICDNGAGYCSNPGIPIGTRKVGSQYRLEDSVTYHCSRGLTLRGSQRRTCQEGGSWSGTEPSCQDSFMYDTPQEVAEAFLSSLTETIEGVDAEDGHGPGEQQKRKIVLDPSGSMNIYLVLDGSDSIGASNFTGAKKCLVNLIEKVASYGVKPRYGLVTYATYPKIWVKVSEADSSNADWVTKQLNEINYEDHKLKSGTNTKKALQAVYSMMSWPDDVPPEGWNRTRHVIILMTDGLHNMGGDPITVIDEIRDLLYIGKDRKNPREDYLDVYVFGVGPLVNQVNINALASKKDNEQHVFKVKDMENLEDVFYQMIDESQSLSLCGMVWEHRKGTDYHKQPWQAKISVIRPSKGHESCMGAVVSEYFVLTAAHCFTVDDKEHSIKVSVGGEKRDLEIEVVLFHPNYNINGKKEAGIPEFYDYDVALIKLKNKLKYGQTIRPICLPCTEGTTRALRLPPTTTCQQQKEELLPAQDIKALFVSEEEKKLTRKEVYIKNGDKKGSCERDAQYAPGYDKVKDISEVVTPRFLCTGGVSPYADPNTCRGDSGGPLIVHKRSRFIQVGVISWGVVDVCKNQKRQKQVPAHARDFHINLFQVLPWLKEKLQDEDLGFL

90-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.