2. Recombinant Proteins
  3. Complement System Biotinylated Proteins
  4. Complement Component 5
  5. Complement Component 5a
  6. Complement C5/C5a Protein, Human (Biotinylated, HEK293, His-Avi)

Complement C5/C5a Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P705144
Handling Instructions Technical Support

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
25 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
200 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
> 200 μg   Get quote  
Synthetic products have potential research and development risk.
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Top Publications Citing Use of Products

  • Technical Parameters

  • Properties

  • Documentation

Species

Human
Source

HEK293
Tag

C-Avi;C-His
Accession

P01031 (Q19-C1676)
Gene ID

727
Molecular Construction
N-term
C5a (Q19-C1676)
Accession # P01031
His-Avi
C-term
Synonyms
C5a; Complement C5; C5; CPAMD4; ECLZB
Predicted Molecular Mass
189.9 kDa.
Molecular Weight

Approximately 75 kDa, 120 kDa and 200 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
Glycosylation
Yes
Purity

Greater than 95% as determined by reducing reducing SDS-PAGE.
Appearance

Lyophilized powder.
Formulation

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping

Room temperature in continental US; may vary elsewhere.
Documentation
Handling Instructions (2659 KB)

Complement C5/C5a Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Complement C5/C5a Protein, Human (Biotinylated, HEK293, His-Avi)
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