Complement C3 Protein, Human (HEK293, hFc)

Complement C3/C3a proteins play a key role in initiating the complement system through processing by C3 convertase in the classical and alternative pathways. Upon activation, C3b covalently binds to the surface, while C3a (anaphylatoxin produced by proteolysis of C3) mediates local inflammation. Complement C3 Protein, Human (HEK293, hFc) is the recombinant human-derived Complement C3 protein, expressed by HEK293, with C-hFc labeled tag.

C3 assumes a pivotal role in the initiation of the complement system, and its processing by C3 convertase constitutes a central reaction in both classical and alternative complement pathways. Following activation, C3b has the capacity to covalently bind to cell surface carbohydrates or immune aggregates through its reactive thioester. Concurrently, the anaphylatoxin C3a, derived from the proteolytic degradation of complement C3, serves as a mediator in local inflammatory processes. In instances of chronic inflammation, C3a acts as a chemoattractant for neutrophils, inducing smooth muscle contraction, increasing vascular permeability, and triggering histamine release from mast cells and basophilic leukocytes.

Human

HEK293

C-hFc

P01024 (S23-N1663)

718

Approximately 75-80 kDa,160-180 kDa & 180 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.