Colicin-E1 Protein, E.coli (Cell-Free, His, Myc)

Colicin-E1, a channel-forming colicin, serves as a transmembrane toxin inducing cytoplasmic membrane depolarization and cellular energy dissipation. It is part of a broader class of polypeptide toxins synthesized by and active against Escherichia coli and related bacterial strains. Colicin-E1 Protein, E.coli (Cell-Free, His, Myc) is the recombinant E. coli-derived Colicin-E1 protein, expressed by E. coli Cell-free , with N-10*His, C-Myc labeled tag. The total length of Colicin-E1 Protein, E.coli (Cell-Free, His, Myc) is 522 a.a., with molecular weight of 64.3 kDa.

Colicin-E1, a representative of the channel-forming colicins, operates as a transmembrane toxin with the capability to induce depolarization of the cytoplasmic membrane, resulting in the dissipation of cellular energy. Belonging to the broader class of colicins, these polypeptide toxins are synthesized by and exhibit activity against Escherichia coli and closely related bacterial strains.

E.coli

E. coli Cell-free

N-10*His;C-Myc

P02978 (M1-I522)

Full length

METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRHNASRTPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNLLNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDVLNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGDWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTINEVLGI

Approximately 66 kDa, based on SDS-PAGE under reducing conditions.

Greater than 85% as determined by reducing SDS-PAGE.