CMKLR1 Protein, Human (Cell-Free, His)

CMKLR1 protein is a receptor for chemerin/RARRES2 and resolvin E1, activating G protein and β-arrestin pathways when RARRES2 interacts. This triggers a cascade of reactions including calcium mobilization, MAP kinase phosphorylation, and PI3K activation, leading to various effects including reduced immune response, adipogenesis, and angiogenesis. CMKLR1 Protein, Human (Cell-Free, His) is the recombinant human-derived CMKLR1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.

The CMKLR1 Protein functions as a receptor for the chemoattractant adipokine chemerin/RARRES2 and the omega-3 fatty acid-derived molecule resolvin E1. Upon interaction with RARRES2, CMKLR1 activates G proteins G(i)/G(o) and beta-arrestin pathways, triggering cellular responses through second messenger pathways like intracellular calcium mobilization, phosphorylation of MAP kinases (MAPK1/MAPK3), TYRO3, MAPK14/P38MAPK, and PI3K. These cascades lead to multifunctional effects, including the reduction of immune responses, enhancement of adipogenesis, and angiogenesis. Resolvin E1, on the other hand, down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. CMKLR1 also plays a positive regulatory role in adipogenesis and adipocyte metabolism. In the context of microbial infection, CMKLR1 acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR, and SIVSM62A) as well as for a primary HIV-1 strain (92UG024-2).

Human

E. coli Cell-free

N-10*His

Q99788 (M1-L373)

1240

Full Length

MRMEDEDYNTSISYGDEYPDYLDSIVVLEDLSPLEARVTRIFLVVVYSIVCFLGILGNGLVIIIATFKMKKTVNMVWFLNLAVADFLFNVFLPIHITYAAMDYHWVFGTAMCKISNFLLIHNMFTSVFLLTIISSDRCISVLLPVWSQNHRSVRLAYMACMVIWVLAFFLSSPSLVFRDTANLHGKISCFNNFSLSTPGSSSWPTHSQMDPVGYSRHMVVTVTRFLCGFLVPVLIITACYLTIVCKLQRNRLAKTKKPFKIIVTIIITFFLCWCPYHTLNLLELHHTAMPGSVFSLGLPLATALAIANSCMNPILYVFMGQDFKKFKVALFSRLVNALSEDTGHSSYPSHRSFTKMSSMNERTSMNERETGML

44 kDa (Monomer) & 88 kDa (Dimer)

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20mM Tris-HCL,0.15M NaCl,0.05%FOS12, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.