CEACAM6 Protein, Human (320a.a, HEK293, His)

CEACAM6 Protein, Human (320a.a, HEK293, His) is the recombinant human-derived CEACAM6 protein, expressed by HEK293, with C-His tag.

CEACAM6 protein, a cell surface glycoprotein, assumes a pivotal role in cell adhesion and tumor progression. Interactions occur in a calcium- and fibronectin-independent manner, mediating both homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules like CEACAM5 and CEACAM8. Particularly, heterophilic interaction with CEACAM8 takes place in activated neutrophils, influencing neutrophil adhesion to cytokine-activated endothelial cells. In the context of tumor progression, CEACAM6 operates as an oncogene by positively regulating cell migration, adhesion to endothelial cells, and invasion. Additionally, it contributes to the metastatic cascade by inducing resistance to anoikis in pancreatic adenocarcinoma and colorectal carcinoma cells. CEACAM6 forms homodimers, engaging in homodimerization via its Ig-like V-type domain, and also forms heterodimers with CEACAM8 through their respective Ig-like V-type domains, highlighting its multifaceted role in cell adhesion and cancer-related processes.

Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM8 (HY-P77629) at 0.5 μg/mL (100 μL/well) on the plate can bind Biotinylated Human CEACAM6. The ED₅₀ for this effect is 1.832 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM8 (HY-P77629) at 0.5 μg/mL (100 μL/well) on the plate can bind Biotinylated Human CEACAM6. The ED₅₀ for this effect is 1.832 μg/mL.

Human

HEK293

C-His

NP_002474.4 (M1-G320)

4680

Full Length of Mature Protein

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.