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  3. CD8A-CD8B Heterodimer Protein, Human (HEK293, His, Flag)

CD8A-CD8B Heterodimer Protein, Human (HEK293, His, Flag)

Cat. No.: HY-P703744
Handling Instructions Technical Support

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD8A&CD8B, His Tag at 2μg/ml (100μl/well) on the plate can bind Anti-CD8 Antibody, hFc Tag. Test result was comparable to standard batch.

Species

Human

Source

HEK293

Tag

C-His;Flag

Accession

P01732(S22-D182)&P10966(L22-P170)

Molecular Weight

Predicted band size: 23.54 kDa; Observed band size: 26-28 kDa. (CD8 alpha) and 28-32 kDa. (CD8 beta)

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CD8A-CD8B Heterodimer Protein, Human (HEK293, His, Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD8A-CD8B Heterodimer Protein, Human (HEK293, His, Flag)
Cat. No.:
HY-P703744
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