1. Recombinant Proteins
  2. CD Antigens
  3. Dendritic Cell CD Proteins Cell Adhesion-related CD Proteins
  4. CD6
  5. CD6 Protein, Rat (HEK293, His)

CD6, a protein, lacks essential conserved residue(s) necessary for propagating feature annotation. CD6 Protein, Rat (HEK293, His) is the recombinant rat-derived CD6 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

CD6, a protein, lacks essential conserved residue(s) necessary for propagating feature annotation. CD6 Protein, Rat (HEK293, His) is the recombinant rat-derived CD6 protein, expressed by HEK293 , with C-His labeled tag.

Background

CD6 lacks conserved residue(s) essential for the propagation of feature annotation.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to rat CD6-His coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/mL PHA, 72.012% cells adhered specifically after 60 minutes at 37°C.

Species

Rat

Source

HEK293

Tag

C-His

Accession

Q812A4 (L18-N396)

Gene ID
Molecular Construction
N-term
CD6 (L18-N396)
Accession # Q812A4
His
C-term
Synonyms
T-cell differentiation antigen CD6; T12; TP120; CD6
AA Sequence

LPPPAPSDQHRNGSIPNMSSDPEEQLGIRLVNGSSRCSGFVQVLLESWEPVCAGHWNRAATEAACNALGCGDLGNFTHLAPPTSERPPGATSRNTSSSRNTTWAGAPTVRCHGANWQLCKVEDYECSSDRRLVWVTCAENQAVRLVDGGSRCAGRVEMLQHGEWGTVCDDTWDLDDANVVCKQLKCGWAVKALAGLHFTPGQGPIHRDQVNCSGTEAYLWDCPGRPGDQYCGHKEDAGVVCSEHQSWRLTGGVDSCEGQVEVYFRGVWSTVCDSEWYPSEAKVLCQSLGCGSEVDRPKGLPHSLAGRMYYSCKGEELTLSTCSWRFNNSNLCSQSLAARVVCSGSQRHPNLSTSEVPSRVPVTIESSVPVSVKDKDSQN

Molecular Weight

Approximately 60-90 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CD6 Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD6 Protein, Rat (HEK293, His)
Cat. No.:
HY-P72924
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