CD39L4/ENTPD5 Protein, Mouse (sf9, His)

The CD39L4/ENTPD5 protein is located in the lumen of the endoplasmic reticulum and plays a crucial role in glycoprotein processing by hydrolyzing nucleoside diphosphates (including GDP, IDP, and UDP). Its enzymatic activity is critical for the clearance of UDP, an end-product feedback inhibitor of UDP-Glc:glycoprotein glucosyltransferase. CD39L4/ENTPD5 Protein, Mouse (sf9, His) is the recombinant mouse-derived CD39L4/ENTPD5 protein, expressed by Sf9 insect cells , with C-His labeled tag.

CD39L4/ENTPD5 protein demonstrates its enzymatic activity by hydrolyzing nucleoside diphosphates, with a notable preference for GDP, IDP, and UDP over ADP and CDP. Particularly, within the endoplasmic reticulum lumen, CD39L4/ENTPD5 plays a vital role in clearing UDP, which acts as an end-product feedback inhibitor of the UDP-Glc:glycoprotein glucosyltransferases. By hydrolyzing UDP, CD39L4/ENTPD5 promotes the regeneration of UDP-glucose in the cytosol through an antiporter mechanism, ensuring a positive regulation of protein reglucosylation. This process is crucial for proper glycoprotein folding and quality control within the endoplasmic reticulum, emphasizing CD39L4/ENTPD5's significance in maintaining cellular functions related to glycoprotein processing.

Mouse

Sf9 insect cells

C-His

Q9WUZ9 (T19-S427)

12499  [NCBI]

Approximately 45 kDa.

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.