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  4. CD30 Ligand/TNFSF8 Protein, Cynomolgus (HEK293, hFc)

CD30 Ligand/TNFSF8 Protein, Cynomolgus (HEK293, hFc)

Cat. No.: HY-P703918
Handling Instructions Technical Support

CD30 Ligand/TNFSF8 Protein, Cynomolgus (HEK293, hFc) is the recombinant cynomolgus-derived CD30 Ligand/TNFSF8 protein, expressed by HEK293, with N-hFc tag.

For research use only. We do not sell to patients.

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Description

CD30 Ligand/TNFSF8 Protein, Cynomolgus (HEK293, hFc) is the recombinant cynomolgus-derived CD30 Ligand/TNFSF8 protein, expressed by HEK293, with N-hFc tag.
Background

CD30 Ligand/TNFSF8 protein, a cytokine, specifically binds to TNFRSF8/CD30, acting as a potent inducer of T-cell proliferation. Operating as a homotrimer, this ligand plays a crucial role in regulating the activation and growth of T-cells, contributing to the dynamic orchestration of immune responses. The interaction between CD30 Ligand and its receptor, TNFRSF8/CD30, highlights its significance as a molecular trigger for the robust expansion of T-cell populations.
Species

Cynomolgus
Source

HEK293
Tag

N-hFc
Accession

G7P2F1 (Q63-D234)
Gene ID

/
Protein Length

Cytoplasmic Domain
Synonyms
tumor necrosis factor (ligand) superfamily; member 8
Predicted Molecular Mass
48.1 kDa
Molecular Weight

Approximately 59 kDa,based on SDS-PAGE under reducing conditions.
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

CD30 Ligand/TNFSF8 Protein, Cynomolgus (HEK293, hFc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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