CD24 Protein, Mouse (HEK293, His)

The CD24 protein may coordinate cellular differentiation of various cell types, particularly in early thymocyte development. Activation of its signaling involves binding of a lectin-like ligand to the CD24 carbohydrate, triggering the release of second messengers from GPI anchors. CD24 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD24 protein, expressed by HEK293, with C-His labeled tag.

CD24 Protein is postulated to play a pivotal role in the cell differentiation processes of various cell types, with a specific implication in early thymocyte development. Its signaling potential is suggested to be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, transduced through the release of second messengers derived from the GPI-anchor. Additionally, CD24 is implicated in modulating B-cell activation responses, indicative of its regulatory influence in immune processes. In conjunction with SIGLEC10, CD24 may participate in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90. This CD24:SIGLEC10 complex is proposed to inhibit HMGB1-mediated tissue damage immune responses, suggesting CD24's role in immune regulation and control of autoimmunity. The multifaceted functions of CD24 underscore its potential as a key player in diverse cellular and immune processes.

Mouse

HEK293

C-His

P24807 (N27-G53)

12484

Approximately 20-40 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.