CCR6 Protein-VLP, Human (HEK293)

The CCR6 protein is a receptor for CCL20 and increases intracellular calcium levels upon ligand binding. Notably, CCR6 also acts as a receptor for non-chemokine ligands such as β-defensins (DEFB1, DEFB4, DEFB4A/B). CCR6 Protein-VLP, Human (HEK293) is the recombinant human-derived CCR6 protein, expressed by HEK293, with tag free.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433.

The CCR6 Protein acts as a receptor for the C-C type chemokine CCL20, transducing signals by increasing intracellular calcium ion levels upon binding to its major ligand. Notably, CCR6 can also function as a receptor for non-chemokine ligands, such as beta-defensins, including DEFB1, DEFB4, and DEFB4A/B. The interaction between CCR6 and DEFB1 is crucial for regulating sperm motility and bactericidal activity. Additionally, CCR6 plays a pivotal role in chemotaxis, being responsible for the migration of dendritic cells, effector/memory T-cells, and B-cells, particularly at skin and mucosal surfaces under various conditions, including inflammation and pathology such as cancer and autoimmune diseases. CCR6-mediated signals are essential for immune responses in the intestinal mucosa, modulating inflammatory responses to tissue insult and trauma. Moreover, CCR6 is indispensable for recruiting pro-inflammatory IL17-producing helper T-cells (Th17) and regulatory T-cells (Treg) to sites of inflammation, influencing thymocyte precursor migration events, B-cell localization in Peyers-patches, and the efficient secondary recall response of memory B-cells. It also positively regulates sperm motility and chemotaxis through its binding to CCL20.

Human

HEK293

Tag Free

P51684 (S2-M374)

1235

Full Length

Solution.

Supplied as 0.2 μm filtered solution of PBS, Arginine, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.