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  4. CCND3 Protein, Human (Biotinylated, MBP, His, Flag)

CCND3 Protein, Human (Biotinylated, MBP, His, Flag)

Cat. No.: HY-P702957
Handling Instructions Technical Support

CCND3 Protein, Human (Biotinylated, MBP, His, Flag) is the recombinant human-derived CCND3, expressed by E.coli , with MBP, His, Flag labeled tag.

For research use only. We do not sell to patients.

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Description

CCND3 Protein, Human (Biotinylated, MBP, His, Flag) is the recombinant human-derived CCND3, expressed by E.coli , with MBP, His, Flag labeled tag.
Background

Cyclin D3 is a regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits retinoblastoma (RB) protein family members, including RB1, thereby regulating the cell cycle during G(1)/S transition. Phosphorylation of RB1 triggers the dissociation of the transcription factor E2F from the RB/E2F complex, leading to the transcription of E2F target genes essential for G(1) phase progression. Cyclin D3-CDK4 complexes serve as key integrators of mitogenic and antimitogenic signals. Additionally, Cyclin D3 forms part of the ternary complex cyclin D3/CDK4/CDKN1B, which is required for the nuclear translocation and activity of the cyclin D-CDK4 complex. Cyclin D3 also exhibits CDK4-independent transcriptional coactivator activity in collaboration with ATF5.
Species

Human
Source

E. coli
Tag

C-6*His;C-Avi;N-MBP
Accession

P30281-1 (M1-L292)
Gene ID

896
Molecular Construction
N-term
MBP
CCND3 (M1-L292)
Accession # P30281-1
6*His
Avi
C-term
Protein Length

Full Length of Isoform-1
Synonyms
G1/S-specific cyclin-D3; CCND3
Predicted Molecular Mass
58 kDa
Molecular Weight

80.3 kDa
Purity

Greater than 85% as determined by reducing SDS-PAGE
Documentation
Handling Instructions (2659 KB)

CCND3 Protein, Human (Biotinylated, MBP, His, Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CCND3 Protein, Human (Biotinylated, MBP, His, Flag)
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