2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Cathepsin
  5. Cathepsin C
  6. Cathepsin C/DPPI Protein, Mouse (HEK293, His)

Cathepsin C/DPPI Protein, Mouse (HEK293, His)

Cat. No.: HY-P704017
SDS
COA
Handling Instructions Technical Support

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

Measured by its ability to cleave 10 μM fluorogenic peptide substrate Gly-Arg-AMC (HY-P4342) that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is >8,000 pmol/min/µg.
Species

Mouse
Source

HEK293
Tag

C-10*His
Accession

P97821 (D25-L462)
Gene ID

13032
Molecular Construction
N-term
CTSC (D25-L462)
Accession # P97821
10*His
C-term
Synonyms
Cathepsin C; cathepsin CEC 3.4.14.1; Cathepsin J; CPPIHMS; CTSC; dipeptidyl peptidase 1; Dipeptidyl peptidase I; Dipeptidyl transferase; dipeptidyl-peptidase I; DPP1; DPPI; DPP-I; JP; JPD; PALS; PLS
Predicted Molecular Mass
51.1 kDa
Molecular Weight

Approximately 52-56 kDa, due to the glycosylation.
Glycosylation
Yes
Purity

Greater than 95% as determined by reducing SDS-PAGE
Appearance

Solution.
Formulation

Supplied as a 0.22 μm filtered solution in 20 mM MES, 150 mM NaCl, pH 5.5.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation

COA (205 KB)

Handling Instructions (2659 KB)

Cathepsin C/DPPI Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Cathepsin C/DPPI Protein, Mouse (HEK293, His)
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