Cathepsin C/DPPI Protein, Human (HEK293, C-His)

Cathepsin C/DPPI Protein, a thiol protease, exhibits dipeptidylpeptidase activity with broad substrate specificity, excluding proline at the P1 position and arginine at the P2 position. Operating as both exopeptidase and endopeptidase, it displays catalytic versatility. Crucially, Cathepsin C/DPPI activates serine proteases like elastase, cathepsin G, and granzymes A and B. Cathepsin C/DPPI Protein, Human (HEK293, C-His) is the recombinant human-derived Cathepsin C/DPPI protein, expressed by HEK293, with C-10*His labeled tag.

Cathepsin C/DPPI Protein functions as a thiol protease, displaying dipeptidylpeptidase activity with a broad substrate specificity encompassing both polar and hydrophobic amino acids. Notably, proline is restricted from the P1 position, and arginine cannot occupy the P2 position of the substrate. This enzyme operates as both an exopeptidase and an endopeptidase, showcasing versatility in its catalytic functions. Of significance, Cathepsin C/DPPI plays a crucial role in the activation of serine proteases, including elastase, cathepsin G, and granzymes A and B.

Measured by its ability to cleave the fluorogenic peptide substrate 10 μM Gly-Arg-AMC (HY-P4342) that incubate at room temperature in kinetic Mode for 5 minutes. The specific activity is 2567.11 pmol/min/µg.

Human

HEK293

C-10*His

P53634-1/NP_001805.3 (D25-L463)

1075

Full Length of Isoform-1 Mature Protein

DTPANCTYLDLLGTWVFQVGSSGSQRDVNCSVMGPQEKKVVVYLQKLDTAYDDLGNSGHFTIIYNQGFEIVLNDYKWFAFFKYKEEGSKVTTYCNETMTGWVHDVLGRNWACFTGKKVGTASENVYVNTAHLKNSQEKYSNRLYKYDHNFVKAINAIQKSWTATTYMEYETLTLGDMIRRSGGHSRKIPRPKPAPLTAEIQQKILHLPTSWDWRNVHGINFVSPVRNQASCGSCYSFASMGMLEARIRILTNNSQTPILSPQEVVSCSQYAQGCEGGFPYLIAGKYAQDFGLVEEACFPYTGTDSPCKMKEDCFRYYSSEYHYVGGFYGGCNEALMKLELVHHGPMAVAFEVYDDFLHYKKGIYHHTGLRDPFNPFELTNHAVLLVGYGTDSASGMDYWIVKNSWGTGWGENGYFRIRRGTDECAIESIAVAATPIPKL

Approximately 55-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution in 20 mM MES, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.