CARM1 Protein, Human (His)

CARM1 Protein, Human (His) is the recombinant human-derived CARM1, expressed by E. coli , with C-10*His labeled tag.

CARM1 is a methyltransferase that mono- and asymmetrically dimethylates arginine residues in proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. It collaborates with EP300/P300 and p160 family histone acetyltransferases to methylate histone H3 at Arg-17 (H3R17me, primarily forming H3R17me2a), activating transcription via chromatin remodeling. During nuclear hormone receptor or TCF7L2/TCF4 activation, CARM1 synergizes with EP300/P300 and p160 acetyltransferases (e.g., NCOA1/SRC1, NCOA2/GRIP1, NCOA3/ACTR) or CTNNB1/beta-catenin to enhance transcription (by similar). In myogenic activation, it acts as a coactivator for MEF2C with NCOA3/ACTR (by similar). It also participates in monocyte inflammatory responses, PPARG-mediated adipocyte differentiation, pre-mRNA splicing regulation, and p53/TP53 transcriptional activation (by similar). CARM1 methylates EP300/P300 at specific arginine sites, disrupting its interaction with CREB, and modifies RNA-binding proteins (e.g., PABPC1, ELAVL1, ELAV4), potentially affecting mRNA stability. Independently of its methyltransferase activity, CARM1 promotes PARP1 recruitment to replication forks, slowing fork progression (PubMed:33412112).

Human

E. coli

C-10*His

Q86X55-3 (A2-S608)

10498

Full Length of Isoform-3 Mature Protein

AAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDSAGIALYSHEDVCVFKCSVSRETECSRVGKQSFIITLGCNSVLIQFATPNDFCSFYNILKTCRGHTLERSVFSERTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNSQFTMGGPAISMASPMSIPTNTMHYGS

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.