Carboxylesterase 1 Protein, Human (HEK293, His)

Carboxylesterase 1 Protein, Human (HEK293, His) is an approximately 60.0 kDa human carboxylesterase 1 protein with a His-flag. Human Carboxylesterase 1 is the key liver microsomal enzyme that takes part in detoxification and metabolism^[1]. 

Carboxylesterases are a family of enzymes that act on a variety of both exogenous (e.g. cocaine) and endogenous (e.g. acyl-CoA esters) substrates. Carboxylesterases are encoded by carboxylesterase (CES or Ces) genes^[1].
Carboxylesterase are defined to hydrolyze ester, amide, or thioester bonds to their corresponding alcohol, amine or thiol and free acid. Human Carboxylesterase 1 (hCES1) is the key liver microsomal enzyme responsible for detoxification and metabolism of a variety of agents^[1]. 

Measured by its ability to hydrolyze p-nitrophenylacetate that incubate at room temperature in kinetic mode for 5 minutes.The specific activity is 10374.757 pmol/min/µg.

Human

HEK293

C-6*His

P23141-3 (H19-E562)

1066  [NCBI]

Partial

HPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHHHHHH

Approximately 60-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

1.Supplied as a 0.22 μm filtered solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 4.0.
2.Supplied as a 0.22 μm filtered solution in 20 mM HAc-NaAc, 150 mM NaCl, pH 4.0, 10% Glycerol.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Victoria Arena de Souza, et al. Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1. PLoS One  [Content Brief]