Carboxylesterase 1 Protein, Human (549a.a, HEK293, His)

Carboxylesterase 1 Protein, Human (549a.a, HEK293, His) is the recombinant human-derived Carboxylesterase 1 protein, expressed by HEK293, with C-His tag.

Carboxylesterase 1 is an enzyme involved in the detoxification of xenobiotics and the activation of ester and amide prodrugs. It hydrolyzes aromatic and aliphatic esters but exhibits no catalytic activity toward amides or fatty acyl-CoA esters. This enzyme hydrolyzes the methyl ester group of cocaine to form benzoylecgonine and catalyzes the transesterification of cocaine to produce cocaethylene. Additionally, Carboxylesterase 1 demonstrates fatty acid ethyl ester synthase activity, esterifying oleic acid to form ethyloleate, and hydrolyzes monoacylglycerides into free fatty acids and glycerol. It also hydrolyzes 2-arachidonoylglycerol and prostaglandins, and converts cellular cholesteryl esters to free cholesterol, promoting reverse cholesterol transport (RCT). Specifically, it facilitates free cholesterol efflux from macrophages to extracellular acceptors and releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into bile. by similar: Its functions also include critical roles in cholesterol metabolism and drug metabolism pathways.

Human

HEK293

C-His

P23141-1 (H19-L567)

1066

Full Length of Isoform-1 Mature Protein

Approximately 55.49 kDa,based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.