2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Isomerases (EC 5)
  5. CA2/Carbonic anhydrase II Protein, Mouse (HEK293, His)

CA2/Carbonic anhydrase II Protein, Mouse (HEK293, His)

Cat. No.: HY-P703734
Handling Instructions Technical Support

CA2/Carbonic anhydrase II Protein, Mouse (HEK293, His) is the recombinant mouse-derived CA2/Carbonic anhydrase II protein, expressed by HEK293, with C-His tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CA2/Carbonic anhydrase II Protein, Mouse (HEK293, His) is the recombinant mouse-derived CA2/Carbonic anhydrase II protein, expressed by HEK293, with C-His tag.
Background

CA2/Carbonic anhydrase II is an enzyme that catalyzes the reversible hydration of carbon dioxide (By similarity). It can also hydrate cyanamide to urea (By similarity), regulates fluid secretion into the anterior chamber of the eye (By similarity), and is essential for bone resorption and osteoclast differentiation (By similarity). Additionally, CA2 contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption (By similar) and stimulates the chloride-bicarbonate exchange activity of SLC26A6 (By similarity).
Species

Mouse
Source

HEK293
Tag

C-8*His
Accession

P00920 (S2-K260)
Protein Length

Full Length of Mature Protein
Synonyms
Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase II; CA-II; Cyanamide hydratase CA2; 4.2.1.69; Ca2; CA-II
Predicted Molecular Mass
30 kDa
Molecular Weight

Approximately 30-35 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

CA2/Carbonic anhydrase II Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CA2/Carbonic anhydrase II Protein, Mouse (HEK293, His)
Cat. No.:
HY-P703734
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.