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  4. CA12/Carbonic anhydrase 12 Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)

CA12/Carbonic anhydrase 12 Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P705171
Handling Instructions Technical Support

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
20 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
100 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
> 100 μg   Get quote  
Synthetic products have potential research and development risk.

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Top Publications Citing Use of Products
  • Technical Parameters

  • Properties

  • Documentation

Species

Cynomolgus

Source

HEK293

Tag

C-Avi;C-His

Accession

A0A2K5TSB2 (A25-G299)

Gene ID

102128063

Molecular Construction
N-term
CA12 (A25-G299)
Accession # A0A2K5TSB2
His-Avi
C-term
Synonyms
Carbonic anhydrase; CA12; Carbonate dehydratase XII; CA-XII
Predicted Molecular Mass
34.5 kDa.
Molecular Weight

Approximately 42-52 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Glycosylation
Yes
Purity

Greater than 60% as determined by reducing Reducing reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from 0.22 μm filtered solution of 50mM Tris,150mM NaCl,PH7.5 with trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CA12/Carbonic anhydrase 12 Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CA12/Carbonic anhydrase 12 Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P705171
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