C-Reactive Protein, Human (His)

C-Reactive Protein, an acute-phase protein, is a marker of inflammation and is produced by the liver. It is used as a diagnostic tool to assess the presence and severity of inflammation in various diseases. C-Reactive Protein's role in inflammation detection and its clinical significance in disease management make it a subject of interest in medical research. C-Reactive Protein, Human (His) is the recombinant human-derived C-Reactive protein, expressed by E. coli , with C-6*His labeled tag.

C-Reactive Protein (CRP) exhibits various functions related to host defense. It facilitates agglutination, bacterial capsular swelling, phagocytosis, and complement fixation by binding to phosphorylcholine in a calcium-dependent manner. CRP is also capable of interacting with DNA and histones, and it may scavenge nuclear material that is released from damaged circulating cells.

1. Immobilized Human CRP-His at 10 μg/mL (100 μl/well) can bind Human CD32a-His Biotinylated by NHS-biotin prior to testing.The ED₅₀ is 7.64 μg/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized Human CD32a at 2 μg/mL (100 μL/well) can bind Biotinylated Human C-Reactive. The ED₅₀ for this effect is 1.182 μg/mL.

Human

E. coli

C-6*His

P02741-1 (Q19-P224)

1401  [NCBI]

QTDMSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGYSIFSYATKRQDNEILIFWSKDIGYSFTVGGSEILFEVPEVTVAPVHICTSWESASGIVEFWVDGKPRVRKSLKKGYTVGAEASIILGQEQDSFGGNFEGSQSLVGDIGNVNMWDFVLSPDEINTIYLGGPFSPNVLNWRALKYEVQGEVFTKPQLWP

Approximately 27 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 2 M Urea, pH 8.0 or PBS, pH 7.4, 0.1% SKL.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.