BRCA1 Protein, Human

BRCA1 Protein, Human is the recombinant human-derived BRCA1, expressed by E. coli, with tag free labeled tag.

BRCA1 is an E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The BRCA1-BARD1 heterodimer coordinates diverse cellular pathways such as DNA damage repair, ubiquitination, and transcriptional regulation to maintain genomic stability. Additionally, it regulates centrosomal microtubule nucleation and is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and G2 phase. BRCA1 is also involved in targeting FANCD2 to sites of DNA damage, inhibits lipid synthesis by binding to inactive phosphorylated ACACA, and contributes to homologous recombination repair (HRR) through its interaction with PALB2. As part of the BRCA1-RBBP8 complex, it regulates CHEK1 activation and controls the G2/M checkpoint following DNA damage via BRCA1-mediated ubiquitination of RBBP8. BRCA1 also functions as a transcriptional activator.

Human

E. coli

Tag Free

P38398 (V1646-P1859)

672

Partial

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.