BLM Protein, Mouse (His-Myc)

BLM is an ATP-dependent DNA helicase that can unwind single- and double-stranded DNA in the 3'-5' direction and actively participates in the DNA replication and repair process. Notably, BLM plays a critical role in 5' end resection during double-strand break repair, recruiting DNA2 to cleave 5'-ssDNA. BLM Protein, Mouse (His-Myc) is the recombinant mouse-derived BLM protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.

BLM, an ATP-dependent DNA helicase, plays a pivotal role in unwinding both single- and double-stranded DNA in a 3'-5' direction. It actively participates in DNA replication and repair processes, contributing to the intricate molecular machinery involved in these essential cellular functions (By similarity). Notably, BLM is a key player in the 5'-end resection of DNA during double-strand break (DSB) repair, where it unwinds DNA and recruits DNA2, which mediates the cleavage of 5'-ssDNA. Additionally, BLM exhibits a negative regulatory role in sister chromatid exchange (SCE) . It demonstrates a capacity to stimulate DNA 4-way junction branch migration and DNA Holliday junction dissolution and displays binding affinity for single-stranded DNA (ssDNA), forked duplex DNA, and DNA Holliday junctions (By similarity). The orchestrated recruitment of BLM to DNA replication forks by the KHDC3-OOEP scaffold, coupled with TRIM25 ubiquitination, underscores its involvement in promoting the restart of stalled replication forks.

Mouse

E. coli

N-10*His;C-Myc

O88700 (I684-Q859)

12144  [NCBI]

INAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQ

Approximately 24.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.