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  4. ENPP-2 Protein, Cynomolgus (HEK293, His)

ENPP-2 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ENPP-2 protein, expressed by HEK293, with N-His tag.

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Description

ENPP-2 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ENPP-2 protein, expressed by HEK293, with N-His tag.
Background

The ENPP-2 Protein hydrolyzes lysophospholipids, specifically lysophosphatidylcholine, to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. It can also act on sphingosylphosphorylcholine, producing sphingosine-1-phosphate, which modulates cell motility. In addition, ENPP-2 Protein has been shown to hydrolyze bis-pNPP, pNP-TMP, and ATP to varying extents. It is involved in various motility-related processes, including angiogenesis and neurite outgrowth, and acts as an angiogenic factor by stimulating the migration of smooth muscle cells and microtubule formation. ENPP-2 Protein is also implicated in melanoma cell migration, potentially through a pertussis toxin-sensitive G protein. It may play a role in parturition induction, as well as in cell proliferation and adipose tissue development. Furthermore, it is required for LPA production in activated platelets and cleaves sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. ENPP-2 Protein exhibits a preference for the sn-1 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF).
Species

Cynomolgus
Source

HEK293
Tag

N-His
Accession

XP_005564040.1 (D49-I863)
Gene ID

102120051
Protein Length

Partial
Synonyms
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; Autotaxin; LysoPLD; Npps2; Pdnp2
Predicted Molecular Mass
96 kDa
Purity

Greater than 85% as determined by reducing SDS-PAGE..
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

ENPP-2 Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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