2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. ARAF Protein, Human (Y301D/Y302D, sf9, GST)

ARAF Protein, Human (Y301D/Y302D, sf9, GST)

Cat. No.: HY-P702927
Handling Instructions Technical Support

ARAF Protein, Human (Y301D/Y302D, sf9, GST) is the recombinant human-derived ARAF protein, expressed by Sf9 insect cells, with N-GST tag.

For research use only. We do not sell to patients.

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Description

ARAF Protein, Human (Y301D/Y302D, sf9, GST) is the recombinant human-derived ARAF protein, expressed by Sf9 insect cells, with N-GST tag.
Background

A-RAF is a protein involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may also regulate the TOR signaling cascade. A-RAF phosphorylates PFKFB2 and serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
Species

Human
Source

Sf9 insect cells
Tag

N-GST
Accession

P10398-1 (K282-P606[Y301D)&(Y302D])
Gene ID

369
Protein Length

Partial
Synonyms
ARAF1; PKS; PKS2
Predicted Molecular Mass
63.3 kDa
Molecular Weight

Approximately 63 kDa,based on SDS-PAGE under reducing conditions.
Purity

Greater than 90% as determined by reducing SDS-PAGE
Documentation
Handling Instructions (2659 KB)

ARAF Protein, Human (Y301D/Y302D, sf9, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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