APLP-2 Protein, Human (HEK293, His)

APLP-2, potentially involved in hemostasis regulation, exhibits inhibitory effects on coagulation factors. Its soluble form may interact with G-protein signaling pathways and bind to DNA. APLP-2 inhibits various proteases and modulates the nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains. It interacts with proteins like CPEB1, DAB2, APBB2/FE65L, and APBB3/FE65L2, suggesting diverse cellular roles. APLP-2 Protein, Human (HEK293, His) is the recombinant human-derived APLP-2 protein, expressed by HEK293 , with C-His labeled tag.

APLP-2, a multifunctional protein, is implicated in the regulation of hemostasis, with its soluble form demonstrating inhibitory properties towards coagulation factors. This protein may engage with cellular G-protein signaling pathways and exhibits binding to the DNA 5'-GTCACATG-3' (CDEI box). APLP-2 showcases diverse inhibitory activities, including trypsin, chymotrypsin, plasmin, factor XIA, and plasma and glandular kallikrein. Moreover, it modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts. In its molecular interactions, APLP-2 engages with CPEB1 and interacts with DAB2 via its NPXY motif, with this interaction being influenced by tyrosine phosphorylation. Additionally, APLP-2 associates with APBB2/FE65L and APBB3/FE65L2, underscoring its involvement in intricate cellular networks.

Human

HEK293

C-8*His

Q06481-1 (G32-S692)

334  [NCBI]

Extracellular Domain

85-100 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.