APAF1 Protein, Human (P. pastoris, His)

APAF1 protein is a key apoptotic component that coordinates cytochrome c-dependent caspase-9 precursor activation and initiates caspase-3 activation and apoptosis. APAF1 forms an apoptotic loop with cytochrome c and dATP, interacts with caspase-9 precursor, and releases mature caspase-9. APAF1 Protein, Human (P. pastoris, His) is the recombinant human-derived APAF1 protein, expressed by P. pastoris , with N-6*His labeled tag.

APAF1 Protein, as an essential component of the apoptosome, orchestrates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), initiating the caspase-3 activation cascade and ultimately leading to apoptosis. The oligomeric form of APAF1 forms a heptameric ring, known as the apoptosome, in the presence of cytochrome c and dATP. Within this complex, APAF1 and pro-caspase-9 interact via their NH2-terminal CARD domains, leading to the release of mature caspase-9. Concurrently, pro-caspase-3 is recruited into the APAF1-pro-caspase-9 complex through interaction with pro-caspase-9, facilitating the activation of caspase-3. Notably, isoform 6 of APAF1 exhibits reduced effectiveness in inducing apoptosis. Additionally, APAF1 interacts with APIP, NAIP/BIRC1, and CIAO2A, contributing to its multifaceted role in the regulation of apoptotic pathways.

Human

P. pastoris

N-6*His

O14727 (S104-L415)

317  [NCBI]

SGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDIL

37.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.