Annexin A7/ANXA7 Protein, Human

Annexin A7 Protein, Human is a 51 kDa multifunctional protein which belongs to the the group A annexin family and contains an extraordinarily long amino terminus. Annexin A7 is a membrane binding protein with diverse properties including voltage sensitive calcium channel activity, ion selectivity and membrane fusion^[1][2].

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins.
Annexin A7 is encoded by ANXA7 gene and is the first isolated annexin, mediating the Ca²⁺-regulated chromaffin granule exocytosis. Annexin 7 has molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. Annexin A7 plays an important role in membrane fusion during exocytosis, localizing predominantly in the cytoplasm and showing a high prevalence in the brain, heart, and skeletal muscle^[1].

Immobilized Human Annexin A7 Protein at 5 μg/mL (100μL/well) can bind Anti-Annexin A7 antibody. The ED₅₀ for this effect is 3.01 ng/mL.

Human

E. coli

Tag Free

P20073-2 (M1-Q466)

310  [NCBI]

Full Length of Isoform-2

MSYPGYPPTGYPPFPGYPPAGQESSFPPSGQYPYPSGFPPMGGGAYPQVPSSGYPGAGGYPAPGGYPAPGGYPGAPQPGGAPSYPGVPPGQGFGVPPGGAGFSGYPQPPSQSYGGGPAQVPLPGGFPGGQMPSQYPGGQPTYPSQPATVTQVTQGTIRPAANFDAIRDAEILRKAMKGFGTDEQAIVDVVANRSNDQRQKIKAAFKTSYGKDLIKDLKSELSGNMEELILALFMPPTYYDAWSLRKAMQGAGTQERVLIEILCTRTNQEIREIVRCYQSEFGRDLEKDIRSDTSGHFERLLVSMCQGNRDENQSINHQMAQEDAQRLYQAGEGRLGTDESCFNMILATRSFPQLRATMEAYSRMANRDLLSSVSREFSGYVESGLKTILQCALNRPAFFAERLYYAMKGAGTDDSTLVRIVVTRSEIDLVQIKQMFAQMYQKTLGTMIAGDTSGDYRRLLLAIVGQ

Approximately 47-52 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 100 mM NaCl, pH 8.0 or 10 mM Tris-HCL, 100 mM NaCl, pH 8.0, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Weihua Ye, et al. Effect of annexin A7 suppression on the apoptosis of gastric cancer cells. Mol Cell Biochem. 2017 May;429(1-2):33-43.  [Content Brief]

  [2]. Hu-Fang Yuan, et al. Downregulation of annexin A7 decreases proliferation, migration, and invasion of gastric cancer cells by reducing matrix metalloproteinase 1 and 9 expression. Am J Transl Res. 2019 May 15;11(5):2754-2764.  [Content Brief]