Animal-Free Enterokinase Protein, Bovine (P. pastoris, His)

Animal-Free Enterokinase Protein, Bovine (P. pastoris, His) is a recombinant Enterokinase protein expressed by P. pastoris with a C-His tag. Enterokinase Protein is a serine protease that plays a crucial role in the digestive system. By specifically cleaving trypsinogen to convert it into active trypsin, Enterokinase Protein initiates the cascade activation of pancreatic zymogens. Enterokinase Protein serves as a key initiator of the intestinal digestive enzyme cascade^[1][2].
Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

Enterokinase is a glycoprotein mainly present in the duodenal and jejunal mucosa. The core role of enterokinase is to activate digestive zymogens, converting trypsinogen into trypsin. The activated trypsin can not only directly decompose proteins but also further activate other zymogens such as chymotrypsinogen, procarboxypeptidase, and proelastase, forming a digestive enzyme cascade reaction to promote protein digestion^[1][2].

Bovine

P. pastoris

C-His

P98072-1 (I801-H1035)

282009

Full Length of Enteropeptidase catalytic light chain

/

Approximately 40 kDa

Greater than 95% as determined by reducing SDS-PAGE

< 1 EU/μg of protein by gel clotting method

• [1]. Mann NS, et al. Enterokinase. Proc Soc Exp Biol Med. 1994 Jun;206(2):114-8.  [Content Brief]

  [2]. Kitamoto Y, et al. Enterokinase, the initiator of intestinal digestion, is a mosaic protease composed of a distinctive assortment of domains. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7588-92.  [Content Brief]