AMSHLP Protein, Human (His)

AMSHLP is a zinc metalloprotease that selectively cleaves "Lys-63" linked polyubiquitin chains and acts as a positive regulator of the TORC1 signaling pathway. It specifically deubiquitinates SESN2, disrupts its interaction with the GATOR2 complex and inhibits SESN2-mediated regulation of TORC1. AMSHLP Protein, Human (His) is the recombinant human-derived AMSHLP protein, expressed by E. coli , with N-6*His labeled tag.

AMSHLP, a zinc metalloprotease, exhibits a selective ability to cleave 'Lys-63'-linked polyubiquitin chains, without affecting 'Lys-48'-linked polyubiquitin chains. Notably, AMSHLP serves as a positive regulator of the TORC1 signaling pathway through its role in the 'Lys-63'-linked deubiquitination of SESN2. This deubiquitination activity impedes the interaction between SESN2 and the GATOR2 complex, thereby contributing to the inhibition of SESN2-mediated TORC1 regulation. These functions underscore AMSHLP's specific involvement in fine-tuning cellular signaling pathways, particularly those associated with TORC1, through precise modulation of 'Lys-63'-linked polyubiquitin chains.

Human

E. coli

N-6*His

Q96FJ0 (D2-R436)

57559

Partial

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.