2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3) Phosphatase
  5. Alkaline Phosphatase
  6. Alkaline Phosphatase/ALPG Protein, Mouse (HEK293, His)

Alkaline Phosphatase/ALPG Protein, Mouse (HEK293, His)

Cat. No.: HY-P700950
Handling Instructions Technical Support

Alkaline Phosphatase/ALPG Proteinas, an enzyme, effectively hydrolyzes various phosphate compounds. Alkaline Phosphatase/ALPG Protein, Mouse (HEK293, His) is the recombinant mouse-derived Alkaline Phosphatase/ALPG protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

Alkaline Phosphatase/ALPG Proteinas, an enzyme, effectively hydrolyzes various phosphate compounds. Alkaline Phosphatase/ALPG Protein, Mouse (HEK293, His) is the recombinant mouse-derived Alkaline Phosphatase/ALPG protein, expressed by HEK293 , with C-His labeled tag.
Background

Alkaline Phosphatase (ALPG) is an enzyme capable of hydrolyzing various phosphate compounds.
Species

Mouse
Source

HEK293
Tag

C-His
Accession

P24823 (V19-S502)
Gene ID
Molecular Construction
N-term
ALPG (V19-S502)
Accession # P24823
His
C-term
Synonyms
Alkaline Phosphatase; Alkaline phosphatase, placental-like; EAP; Alpg; Akp5; Alppl2
Molecular Weight

55-70 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Alkaline Phosphatase/ALPG Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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