2. Recombinant Proteins
  3. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators Biotinylated Proteins
  4. TGF-beta Superfamily Receptor Serine/Threonine Kinases Serine/Threonine Kinase Proteins
  5. Activin/Inhibins Receptor
  6. ALK-7
  7. ALK-7 Protein, Human (Biotinylated, HEK293, His, Avi)

ALK-7 Protein, Human (Biotinylated, HEK293, His, Avi)

Cat. No.: HY-P704149
Handling Instructions Technical Support

ALK-7 Protein, Human (Biotinylated, HEK293, His, Avi) is the recombinant human-derived ALK-7 protein, expressed by HEK293, with C-His & C-Avi tag.

For research use only. We do not sell to patients.

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Description

ALK-7 Protein, Human (Biotinylated, HEK293, His, Avi) is the recombinant human-derived ALK-7 protein, expressed by HEK293, with C-His & C-Avi tag.
Background

ALK-7 is a serine/threonine protein kinase that forms a receptor complex upon ligand binding. The receptor complex consists of 2 type II and 2 type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors, which autophosphorylate and subsequently bind and activate SMAD transcriptional regulators SMAD2 and SMAD3. ALK-7 serves as a receptor for activin AB, activin B, activin E, and NODAL. Upon NODAL binding, activation of ALK-7 leads to increased apoptosis and reduced proliferation by suppressing AKT signaling and activating the Smad2-dependent signaling pathway in pancreatic beta-cells, trophoblasts, epithelial cells, or neuronal cells. Additionally, ALK-7 acts as a positive regulator for macrophage activation, partially through down-regulation of PPARG expression (by similarity).
Species

Human
Source

HEK293
Tag

C-Avi;C-10*His
Accession

Q8NER5 (L22-E113)
Gene ID

130399
Molecular Construction
N-term
ALK-7 (L22-E113)
Accession # Q8NER5
10*His
Avi
C-term
Protein Length

Extracellular Domain
Synonyms
Activin receptor type IC; ACTR-IC; ACVRLK7; ALK7
AA Sequence

LSPGLKCVCLLCDSSNFTCQTEGACWASVMLTNGKEQVIKSCVSLPELNAQVFCHSSNNVTKTECCFTDFCNNITLHLPTASPNAPKLGPME

Predicted Molecular Mass
13.7 kDa
Molecular Weight

Approximately 20-40 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Purity

Greater than 95% as determined by Bis-Tris PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

ALK-7 Protein, Human (Biotinylated, HEK293, His, Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ALK-7 Protein, Human (Biotinylated, HEK293, His, Avi)
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