Aconitase 2/ACO2 Protein, Mouse (sf9, His-GST)

Aconitase 2 (ACO2) protein catalyzes the isomerization of citric acid to isocitrate through the intermediate aconitate, playing a crucial role in cellular metabolism. This enzyme activity is a key step in the tricarboxylic acid (TCA) cycle, a central pathway for energy production and generation of metabolic intermediates. Aconitase 2/ACO2 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived Aconitase 2/ACO2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Aconitase 2 (ACO2) is an enzyme that plays a crucial role in the tricarboxylic acid (TCA) cycle by catalyzing the isomerization of citrate to isocitrate via the intermediate cis-aconitate. This reversible reaction is essential for the efficient metabolism of carbohydrates and the production of energy in the form of NADH. ACO2 acts as a key regulator in coordinating the flow of carbon through the TCA cycle, contributing to the generation of reducing equivalents and precursors for various biosynthetic pathways. The isomerization of citrate to isocitrate, facilitated by ACO2, represents a fundamental step in cellular energy production and metabolic homeostasis.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

Sf9 insect cells

N-His;N-GST

Q99KI0 (Q28-Q780)

11429  [NCBI]

Full Length of Mature Protein

Approximately 100 kDa,based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Glycerol, 0.5 mM GSH, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping with dry ice.