1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. ABHD4 Protein, Human (sf9, His)

ABHD4 is a lysophospholipase that acts selectively on N-acylphosphatidylethanolamines (NAPE), which is essential for the biosynthesis of N-acylphosphatidylethanolamines such as anandamide. It hydrolyzes the sn-1 and sn-2 acyl chains in NAPE to generate glycerophosphate-N-acylethanolamine (GP-NAE), which is an important intermediate in the production of N-acylethanolamine. ABHD4 Protein, Human (sf9, His) is the recombinant human-derived ABHD4 protein, expressed by Sf9 insect cells , with N-His labeled tag.

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Description

ABHD4 is a lysophospholipase that acts selectively on N-acylphosphatidylethanolamines (NAPE), which is essential for the biosynthesis of N-acylphosphatidylethanolamines such as anandamide. It hydrolyzes the sn-1 and sn-2 acyl chains in NAPE to generate glycerophosphate-N-acylethanolamine (GP-NAE), which is an important intermediate in the production of N-acylethanolamine. ABHD4 Protein, Human (sf9, His) is the recombinant human-derived ABHD4 protein, expressed by Sf9 insect cells , with N-His labeled tag.

Background

ABHD4, a lysophospholipase, exhibits selectivity for N-acyl phosphatidylethanolamine (NAPE) and plays a vital role in the biosynthesis of N-acyl ethanolamines, such as the endocannabinoid anandamide. It achieves this by hydrolyzing the sn-1 and sn-2 acyl chains from NAPE, generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate crucial for N-acyl ethanolamine biosynthesis. ABHD4 demonstrates activity across a range of substrates with saturated, monounsaturated, and polyunsaturated N-acyl chains, contributing to the diversity of N-acyl ethanolamines produced. Notably, it exhibits no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylserine.

Species

Human

Source

Sf9 insect cells

Tag

N-His

Accession

Q8TB40 (M1-D342)

Gene ID
Molecular Construction
N-term
His
ABHD4 (M1-D342)
Accession # Q8TB40
C-term
Synonyms
(Lyso)-N-acylphosphatidylethanolamine lipase; Alpha/beta-hydrolase 4; ABHD4
Molecular Weight

Approximately 40 kDa.

Purity

Greater than 82% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

ABHD4 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ABHD4 Protein, Human (sf9, His)
Cat. No.:
HY-P76133
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