2. Recombinant Proteins
  3. Viral Proteins
  4. A29 Protein, Monkeypox virus (His)

A29 Protein, Monkeypox virus (His) is the recombinant virus-derived A29 protein, expressed by E. coli, with N-His tag.

For research use only. We do not sell to patients.

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Description

A29 Protein, Monkeypox virus (His) is the recombinant virus-derived A29 protein, expressed by E. coli, with N-His tag.
Background

Mpox virus (MPXV) A29 protein is a small protein with a molecular weight of approximately 14 kDa. Vaccinia virus (MPXV), formerly known as monkeypox virus, became a global epidemic in 2022. Most seroprevalence or vaccine studies use live MPXV (or vaccinia virus [VACV]) or inactivated MPXV (or VACV) culture lysates for serological testing, but MPXV culture can only be performed in biosafety level 3 (BSL-3) facilities.
Species

Virus
Source

E. coli
Tag

N-8*His
Accession

Q90188 (M1-E110)
Gene ID

/
Protein Length

Full Length
Synonyms
A29L; IMV surface; 14 kDa protein; 14kDa fusion;
Predicted Molecular Mass
13.7 kDa
Molecular Weight

Approximately 13.7 kDa,based on SDS-PAGE under reducing conditions.
Purity

Greater than 95% as determined by Bis-Tris PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

A29 Protein, Monkeypox virus (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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A29 Protein, Monkeypox virus (His)
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