2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Protein Kinase Inhibitor Peptide (PKI)
  5. 14-3-3 zeta Protein, Mouse (sf9, His)

14-3-3 zeta Protein, Mouse (sf9, His) is the recombinant mouse-derived 14-3-3 zeta, expressed by Sf9 insect cells, with His labeled tag.

For research use only. We do not sell to patients.

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Description

14-3-3 zeta Protein, Mouse (sf9, His) is the recombinant mouse-derived 14-3-3 zeta, expressed by Sf9 insect cells, with His labeled tag.
Background

14-3-3 zeta is an adapter protein involved in regulating a broad spectrum of general and specialized signaling pathways. It binds to numerous partners via recognition of phosphoserine or phosphothreonine motifs, typically modulating the activity of bound partners. The protein promotes cytoplasmic retention and inactivation of phosphorylated TFEB. By similarity, it enhances ARHGEF7 activity on RAC1 to induce lamellipodia and membrane ruffle formation, and in neurons, regulates spine maturation through ARHGEF7 activity modulation (By similarity).
Species

Mouse
Source

Sf9 insect cells
Tag

N-His
Accession

P63101 (M1-N245)
Gene ID

22631
Protein Length

Full Length
Synonyms
14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1 (KCIP-1); SEZ-2; Ywhaz
Predicted Molecular Mass
31 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Documentation
SDS (251 KB)

COA (219 KB)

Handling Instructions (2659 KB)

14-3-3 zeta Protein, Mouse (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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14-3-3 zeta Protein, Mouse (sf9, His)
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