Perfluorohexane (Synonyms: AF-0150)

Cat. No.: HY-109540 Purity: 98.62%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Perfluorohexane (AF-0150) is a perfluorocarbon. Perfluorohexane is also an enhancer of high-intensity focused ultrasound (HIFU) therapy. Perfluorohexane increases HO-1 and IL-10. Perfluorohexane exerts an antioxidant effect. Perfluorohexane alleviates lung injury induced by LPS (HY-D1056A1). Perfluorohexane improves the efficiency and precision of treatment and reduces damage to surrounding normal tissues. Perfluorohexane can be used in the study of acute lung injury.

For research use only. We do not sell to patients.

Perfluorohexane Chemical Structure

CAS No. : 355-42-0

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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•Related Screening Libraries:

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•Related Proteins:

View All Heme Oxygenase (HO) Isoform Specific Products:

View All Isoforms

HO-1 HO-2

View All Interleukin Related Isoform Specific Products:

View All Isoforms

IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Perfluorohexane (12-36 h) shows no cytotoxicity to human mesothelial cells, does not induce cell activation, and does not significantly affect the expression of cell adhesion molecules ICAM-1, VCAM-1 and surfactant protein A^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Perfluorohexane (10 mL/kg; i.p.; every 2 h) attenuates Lipopolysaccharide (HY-D1056A1)-induced lung injury in rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats (4-6 months old, weighing 250-300 g); lipopolysaccharide-induced acute lung injury model^[3]
Dosage:	10 mL/kg
Administration:	Intraperitoneal injection; every 2 h.
Result:	Decreased lung wet/dry weight ratio, malondialdehyde content, and myeloperoxidase activity. Reduced tumor necrosis factor-α level and increased interleukin-10 level in bronchoalveolar lavage fluid. Induced the expression of heme oxygenase-1.

Molecular Weight

338.04

Formula

C₆F₁₄

CAS No.

355-42-0

Appearance

Liquid (Density: 1.665 g/cm³)

Color

Colorless to light yellow

SMILES

FC(F)(C(F)(C(F)(C(F)(C(F)(C(F)(F)F)F)F)F)F)F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (295.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9582 mL	14.7911 mL	29.5823 mL
5 mM	0.5916 mL	2.9582 mL	5.9165 mL
10 mM	0.2958 mL	1.4791 mL	2.9582 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.62%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (246 KB)

Handling Instructions (2659 KB)

References

[1]. Haufe D, et al. Effect of perfluorohexane on the expression of cellular adhesion molecules and surfactant protein A in human mesothelial cells in vitro. Artif Cells Blood Substit Immobil Biotechnol. 2011 Aug;39(4):239-46. [Content Brief]

[2]. Wang X, et al. Perfluorohexane‐encapsulated mesoporous silica nanocapsules as enhancement agents for highly efficient high intensity focused ultrasound (HIFU)[J]. Advanced Materials, 2012, 6(24): 785-791. [Content Brief]

[3]. Ge ZJ, et al. Systemic perfluorohexane attenuates lung injury induced by lipopolysaccharide in rats: the role of heme oxygenase-1. Pharmacol Rep. 2010 Jan-Feb;62(1):170-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9582 mL	14.7911 mL	29.5823 mL	73.9557 mL
	5 mM	0.5916 mL	2.9582 mL	5.9165 mL	14.7911 mL
	10 mM	0.2958 mL	1.4791 mL	2.9582 mL	7.3956 mL
	15 mM	0.1972 mL	0.9861 mL	1.9722 mL	4.9304 mL
	20 mM	0.1479 mL	0.7396 mL	1.4791 mL	3.6978 mL
	25 mM	0.1183 mL	0.5916 mL	1.1833 mL	2.9582 mL
	30 mM	0.0986 mL	0.4930 mL	0.9861 mL	2.4652 mL
	40 mM	0.0740 mL	0.3698 mL	0.7396 mL	1.8489 mL
	50 mM	0.0592 mL	0.2958 mL	0.5916 mL	1.4791 mL
	60 mM	0.0493 mL	0.2465 mL	0.4930 mL	1.2326 mL
	80 mM	0.0370 mL	0.1849 mL	0.3698 mL	0.9244 mL
	100 mM	0.0296 mL	0.1479 mL	0.2958 mL	0.7396 mL

Perfluorohexane Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Perfluorohexane355-42-0AF-0150AF0150AF 0150Heme Oxygenase (HO)Interleukin RelatedHeme OxygenaseILHigh energy focused ultrasoundtissue necrosisenhancerultrasound imagingInhibitorinhibitorinhibit

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